Anti-RNASEH2C Rabbit Polyclonal Antibody for WB and ELISA - Q8TDP1

Core Product Specifications and Parameters

Biological Background: RNASEH2C Function and Localization

• RNASEH2C is the non-catalytic subunit of the RNase H2 complex, an endonuclease that specifically degrades the RNA component of RNA:DNA hybrids.
• The RNase H2 complex plays a critical role in DNA replication, potentially mediating the removal of Okazaki fragment RNA primers during lagging-strand synthesis.
• It is also responsible for the excision of single misincorporated ribonucleotides from DNA duplexes, maintaining genome integrity.
• Encoded by the RNASEH2C gene (also known as AYP1), mutations in this subunit are associated with Aicardi-Goutières syndrome (AGS3), a severe neuroinflammatory disorder.
• Subcellular localization is primarily nuclear, consistent with its role in DNA replication and repair.
• The protein is widely expressed across human tissues and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human RNASEH2C protein, which may be useful for detecting both native and denatured forms in Western blot applications.
• For ELISA, consider using the antibody as a capture or detection reagent with recombinant or native RNase H2 complex; validate pairing with other subunits if needed.
• Due to potential cross-reactivity with other species, it is advisable to verify specificity in the relevant sample system, especially if using non-human models.
• Optimization of antibody dilution and blocking conditions is recommended, as performance may vary depending on sample type and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, providing validated antibodies, optimized monoclonals and polyclonals, bulk ancillary reagents, and sourcing for rare targets like RNASEH2C. For researchers and diagnostic developers focusing on nucleic acid metabolism, genome stability, or Aicardi-Goutières syndrome, this anti-RNASEH2C antibody serves as a reliable tool, backed by our integrated supply chain and technical support.