Anti-RNASEH2A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75792

Core Product Specifications and Parameters

Biological Background: RNASEH2A Function and Localization

• RNASEH2A encodes the catalytic subunit of ribonuclease HII (RNase HII), an enzyme that specifically degrades the RNA strand in RNA:DNA hybrids.
• It plays an essential role in DNA replication by removing lagging-strand Okazaki fragment RNA primers, ensuring accurate DNA synthesis.
• The enzyme also mediates the excision of single ribonucleotides erroneously incorporated into DNA, maintaining genome stability.
• RNASEH2A is localized to the nucleus, consistent with its function in DNA metabolic processes.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics studies.
• Mutations in RNASEH2A are causative for Aicardi-Goutieres syndrome type 4 (AGS4), a severe autoimmune disorder characterized by aberrant type I interferon signaling.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human RNASEH2A (amino acids 1-299), which may provide broad epitope recognition; however, epitope-specific responses should be validated in the target sample system.
• Suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. For WB, the predicted molecular weight is approximately 33 kDa; consider using nuclear extracts to enrich the target signal.
• For IF/ICC, typical nuclear localization can be confirmed using appropriate DNA counterstains; ensure adequate fixation and permeabilization for nuclear antigen access.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to titrate and optimize for each application and include relevant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. The RNASEH2A target, central to DNA replication fidelity and genetic disorders such as Aicardi-Goutieres syndrome, is accessible through our extensive portfolio of validated antibodies. Beyond this product, we supply optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and offer rare target raw-material sourcing to accelerate your assay development.