Anti-RNASEH1 Rabbit Polyclonal Antibody for WB and ELISA - O60930

Core Product Specifications and Parameters

Biological Background: RNASEH1 Function and Localization

• RNASEH1 (also known as Ribonuclease H1, Ribonuclease H type II, or RNH1) is an endonuclease encoded by the human RNASEH1 gene (GeneID: 246243).
• It specifically degrades the RNA strand of RNA-DNA hybrids, a critical function for resolving co-transcriptional R-loops and maintaining genome stability. Related references: PMID:10497183
• RNASEH1 participates in RNA polymerase II transcription termination by cleaving R-loop structures that form at G-rich pause sites downstream of poly(A) signals, thereby facilitating proper gene expression. Related references: PMID:21700224
• The enzyme is ubiquitously expressed across human tissues and is primarily localized in the cytoplasm.
• RNASEH1 is a metal-dependent hydrolase that requires magnesium ions for catalytic activity.
• Mutations in RNASEH1 are associated with progressive external ophthalmoplegia with mitochondrial DNA deletions (PEOB2), highlighting its role in mitochondrial DNA maintenance.

Experimental Guidance and Technical Tips

• For Western blotting, consider testing a range of antibody dilutions on whole cell lysates from human, mouse, or rat samples; the predicted molecular weight of RNASEH1 is ~32kDa.
• In ELISA applications, this polyclonal antibody may be used as a capture or detection reagent after appropriate pairing and titration validation.
• Due to its polyclonal nature, the antibody likely recognizes multiple epitopes within the immunogen sequence (amino acids 1–286), potentially enhancing sensitivity across species and applications.
• Always include appropriate controls (e.g., lysates from known positive/negative cell lines or tissues) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering a seamless sourcing experience from discovery to clinical application. Our portfolio includes validated polyclonal antibodies like this anti-RNASEH1 antibody, alongside monoclonal antibodies, antigen proteins, and ancillary reagents for RNA metabolism research. With expertise in rare target sourcing and bulk supply, we help accelerate your diagnostic assay development.