Anti-RNASEH1 Monoclonal Antibody for WB, ELISA - O60930

Core Product Specifications and Parameters

Biological Background: RNASEH1 Function and Localization

• RNASEH1 (Ribonuclease H1) is an endonuclease that specifically cleaves the RNA strand of RNA-DNA hybrids (PubMed:10497183). Related references: PMID:10497183
• It facilitates RNA polymerase II transcription termination by degrading R-loop structures formed at G-rich pause sites downstream of poly(A) signals (PubMed:21700224). Related references: PMID:21700224
• The enzyme requires divalent metal ions, such as magnesium, for its catalytic function.
• RNASEH1 is predominantly localized in the cytoplasm.
• It is ubiquitously expressed across human tissues.
• Pathogenic variants in RNASEH1 are associated with primary mitochondrial disease and progressive external ophthalmoplegia.
• The protein has a predicted molecular weight of approximately 32 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 140–286) covers a central conserved region of RNASEH1, which likely contributes to the observed cross-reactivity among human, mouse, and rat.
• For Western blot, whole cell lysates from HeLa, HEK293, or NIH/3T3 cells may be used; a band around 32 kDa is expected.
• Consider validating antibody specificity using RNASEH1 knockout cell lines or siRNA-mediated knockdown.
• For ELISA applications, titrate the antibody concentration to determine optimal signal-to-noise ratio for your specific assay format.
• Since RNASEH1 is involved in R-loop resolution, this antibody can be used to study R-loop dynamics when combined with established R-loop detection methods (e.g., S9.6 antibody).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with a comprehensive portfolio of IVD raw materials, including this RNASEH1 monoclonal antibody for DNA/RNA hybrid metabolism studies. Beyond individual antibodies, we supply matched pairs, recombinant proteins, and bulk ancillary reagents to streamline your assay development from early-stage research to clinical application. Our sourcing expertise extends to rare and emerging targets, ensuring you have reliable access to the critical tools needed for innovative diagnostic solutions.