Anti-RNASE3 Rabbit Polyclonal Antibody for WB, ELISA - P12724

Core Product Specifications and Parameters

Biological Background: RNASE3 Function and Localization

• RNASE3 (also known as eosinophil cationic protein, ECP) is a secreted protein that functions as a cytotoxin and helminthotoxin, with low-efficiency ribonuclease activity.
• It disrupts bacterial membranes, leading to cytoplasmic membrane depolarization, outer membrane detachment, and loss of cellular content in both Gram-negative and Gram-positive bacteria.
• RNASE3 is released from activated eosinophils and contributes to host defense against parasitic helminths and bacterial infections.
• The protein contains several disulfide bonds typical of the RNase A superfamily and undergoes post-translational modifications including glycosylation and tyrosine nitration.
• Subcellularly, RNASE3 is processed through the secretory pathway and is found in eosinophil granules and extracellular fluids.
• Its ribonuclease activity, although weak, may contribute to antiviral activity and RNA degradation in the inflammatory milieu.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 100–160 of human RNASE3; this region shows high homology with rat RNASE3, consistent with observed cross-reactivity. Verify reactivity in other species via sequence alignment.
• For Western blotting, expect a major band at ~18 kDa; additional bands from glycosylation or aggregation may be observed. Use reducing conditions and validate with appropriate controls.
• In ELISA, use recombinant RNASE3 or native protein from eosinophil lysates as coating antigen. Optimize coating buffer and blocking to minimize background.
• Polyclonal antibodies may exhibit lot-to-lot variability; consider validating each new lot in your specific assay setup for consistent performance.
• RNASE3 can be detected in serum, plasma, or cell culture supernatants. Include positive and negative controls (e.g., eosinophil vs. neutrophil lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of IVD raw materials, supporting diagnostic manufacturers, research labs, and clinical institutions from early development to commercialization. This anti-RNASE3 polyclonal antibody is ideal for immunoassay development targeting eosinophil-associated disorders and innate immunity. In addition to validated antibodies, CamelBio offers a broad range of antigens, antibody pairs, and ancillary reagents, with expertise in sourcing rare targets to accelerate your diagnostic projects.