Anti-RNASE2 Rabbit Polyclonal Antibody for WB - O35291

Core Product Specifications and Parameters

Biological Background: RNASE2 Function and Localization

• RNASE2, also known as non-secretory ribonuclease or eosinophil cationic-type ribonuclease 4 (MR-4), is a member of the ribonuclease A superfamily (UniProt O35291).
• It functions as a pyrimidine-specific endonuclease with a slight preference for uridine (U), catalyzing the cleavage of RNA.
• RNASE2 exhibits cytotoxic and helminthotoxic activities, playing a role in host defense against parasites and other pathogens.
• The protein localizes to lysosomes and cytoplasmic granules, consistent with its role in degradation of RNA and antimicrobial responses.
• RNASE2 is a glycoprotein and contains disulfide bonds, nitration sites, and a signal peptide, common features of ribonucleases (KEYWORDS).
• The mouse Rnase2 gene (Ear4) encodes a protein of ~18 kDa, corresponding to the predicted molecular weight (UniProt: 17723 Da).

Experimental Guidance and Technical Tips

• For Western blotting (WB), consider using a dilution range optimized for the detection of the ~18 kDa RNASE2 band in relevant tissue or cell lysates; validate with positive controls such as eosinophil-enriched samples.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), note the enzyme's lysosomal and granular localization; adequate permeabilization and antigen retrieval may be critical for tissue sections.
• For ELISA, pairing with a suitable capture antibody may be necessary; ensure recombinant protein or native RNASE2 is used for standard curve generation.
• Given the reported cross-reactivity with human, mouse, and rat, validate the antibody's performance in your species of interest under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibodies and ancillary reagents. This RNASE2 polyclonal antibody supports research into eosinophil biology, ribonuclease function, and parasitic infection models. CamelBio’s comprehensive portfolio covers optimized antibody pairs, monoclonal and polyclonal antibodies, and bulk reagent supply for research and IVD development.