Anti-RNASE2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P10153

Core Product Specifications and Parameters

Biological Background: RNASE2 Function and Localization

• RNASE2, also known as eosinophil-derived neurotoxin (EDN), RNase UpI-2, ribonuclease 2, and ribonuclease US, is a non-secretory ribonuclease belonging to the pancreatic ribonuclease family.
• It functions as a pyrimidine-specific endonuclease with a slight preference for uridine, and exhibits a variety of biological activities including cytotoxicity, helminthotoxicity, and selective chemotaxis for dendritic cells.
• The protein contains a signal peptide and is a glycoprotein with disulfide bonds; it is subject to nitration, which may modulate its activity.
• Subcellular localization includes lysosomes and cytoplasmic granules, consistent with its roles in degradation and immune cell effector functions.
• Tissue expression is found in liver, lung, spleen, leukocytes, and body fluids, indicating broad distribution and potential roles in both tissue remodeling and host defense.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering amino acids 28–161, which corresponds to the mature protein after signal peptide cleavage. This design may enhance specificity for the native, processed form.
• For Western blot (WB), a band at approximately 18 kDa is expected; consider additional bands from glycosylation or dimerization under non-reducing conditions.
• In immunofluorescence on paraffin-embedded sections (IF-P), antigen retrieval may be necessary; validate in known expressing tissues such as liver or leukocyte-rich samples.
• For ELISA, the antibody can be considered as a capture or detection reagent; pairing with another clone or antibody may be required for sandwich formats.
• Cross-reactivity with mouse RNASE2 is reported; verify performance in the relevant experimental model, as sequence homology is high but epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined sourcing channel for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This RNASE2 antibody supports research and assay development in eosinophil biology, inflammatory diseases, and ribonuclease-related diagnostics. Beyond individual reagents, CamelBio assists in rare target raw-material sourcing and offers technical services covering concept-to-clinic stages, reinforcing your project with reliable supply and expert support.