Anti-RNASE13 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5GAN3

Core Product Specifications and Parameters

Biological Background: RNASE13 Function and Localization

• RNASE13 encodes a probable inactive ribonuclease-like protein 13, a member of the ribonuclease A superfamily.
• The protein does not exhibit any detectable ribonuclease activity under standard conditions.
• It contains a predicted N-terminal signal peptide, directing it to the secretory pathway.
• RNASE13 is annotated as a glycoprotein and has been identified in large-scale proteomics studies.
• The gene is also known by aliases RAL1 and HEL-S-86p.
• Subcellular fractionation and secretion assays suggest extracellular localization.
• The unprocessed precursor has a calculated molecular weight of approximately 17.8 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human RNASE13 sequence (amino acids 1–156), potentially enabling recognition of native and denatured protein forms.
• For Western blot, consider using whole-cell lysates or tissue extracts under reducing conditions; the band should appear near 18 kDa. Include positive and negative controls to validate specificity.
• In immunohistochemistry (paraffin sections), an appropriate antigen retrieval method (e.g., heat-mediated citrate buffer) may improve staining; test in tissues known to express RNASE13.
• ELISA experiments may benefit from coating with recombinant RNASE13 or screening of biological fluids such as serum or culture supernatants, given the protein's secretory nature.
• Cross-reactivity with mouse RNASE13 is indicated by the supplier; verify in the relevant mouse model systems for your study.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw material sourcing, including validated antibodies, antigen reagents, and ancillary products to support your diagnostic development from concept to clinic. This anti-RNASE13 rabbit polyclonal antibody is a valuable tool for investigating secreted ribonuclease-like proteins in both research and immunoassay contexts. Through our extensive supply network, we offer bulk ordering, rare-target sourcing, and technical consultation to streamline your procurement workflow.