Anti-RNASE1 Polyclonal Antibody for WB, ELISA - P07998

Core Product Specifications and Parameters

Biological Background: RNASE1 Function and Localization

• Ribonuclease pancreatic (RNASE1), also known as HP-RNase, RIB-1, RNase UpI-1, RNase 1, and RNase A, is encoded by the RNASE1 gene (aliases RIB1, RNS1).
• It functions as an endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides, acting on both single-stranded and double-stranded RNA.
• Subcellular localization: Secreted, consistent with its role in extracellular RNA degradation.
• Tissue specificity: Expressed in the pancreas and other tissues and body fluids, indicating additional physiological functions beyond digestion.
• Annotated as a glycoprotein with disulfide bonds; also classified as a hydrolase, lyase, nuclease, and reference proteome.
• The calculated molecular weight from UniProt is approximately 17.6 kDa, aligning with the observed ~18 kDa band in denaturing gels.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29–156 of human RNASE1, likely representing the mature secreted form after signal peptide cleavage. Users should validate detection of endogenous protein in relevant sample types (e.g., cell supernatants, pancreatic extracts).
• For Western blotting, an ~18 kDa band is expected under reducing conditions. Due to the presence of disulfide bonds, consider including reducing agents in sample preparation.
• This antibody may cross-react with mouse RNase1; validate specificity if using mouse-derived samples. For ELISA, use recombinant RNASE1 protein as a quantitative standard and optimize coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, providing diagnostic manufacturers and research institutes with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-RNASE1 polyclonal antibody, along with complementary RNASE1-related reagents, can be reliably procured through CamelBio to support your assay development and translational research needs.