Anti-pro Caspase-1 + p10 + p12 Rabbit mAb for WB, IP, ELISA - P29466

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1 (CASP1; also known as IL-1BC, ICE, p45) functions as a thiol protease that cleaves the precursors of interleukin-1 beta (IL1B) and interleukin 18 (IL18) to produce active inflammatory cytokines.
  Related references: PMID:15326478, PMID:15498465, PMID:1574116

• It initiates pyroptosis, a lytic cell death pathway, through cleavage of gasdermin D (GSDMD); this cleavage relies on an exosite interface rather than strict consensus site recognition.
  Related references: PMID:26375003, PMID:32051255, PMID:32109412, PMID:32553275

• For canonical substrates, caspase-1 recognizes a tetrapeptide motif with Asp at P1, but GSDMD recognition is exosite-dependent.
  Related references: PMID:15498465, PMID:1574116, PMID:7876192

• Upon bacterial infection, caspase-1 cleaves and activates caspase-7 (CASP7) to promote plasma membrane repair.
  Related references: PMID:22464733

• During DNA virus infection, caspase-1 cleaves and inactivates cGAS, thereby modulating antiviral innate immunity.
  Related references: PMID:28314590

• In apoptotic cells, it processes sphingosine kinase 2 (SPHK2), which is then released and retains enzymatic activity extracellularly.
  Related references: PMID:20197547

• Caspase-1 localizes mainly to the cytoplasm and cell membrane; it is highly expressed in spleen and lung and absent in brain. It undergoes proteolytic activation from its zymogen form and is subject to phosphorylation, isopeptide bond formation, and ubiquitin-like modifications.

Experimental Guidance and Technical Tips

• The antibody is raised against recombinant protein, likely recognizing both the pro-form (45 kDa) and cleaved p10/p12 subunits; confirm band patterns in the relevant sample system.
• For Western blotting, consider the protein weight of 45 kDa for pro-Caspase-1, and validate the presence of any processed forms.
• Reported cross-reactivity with human, mouse, and rat enables multi-species studies; however, compatibility should be verified in-house for each application.
• This rabbit monoclonal antibody is suitable for immunoprecipitation (IP) and ELISA; assay conditions may require optimization for specific experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline your IVD raw material procurement with a one-stop platform offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Whether you require Caspase-1–related research tools for inflammation and pyroptosis studies or rare target sourcing, our supply capabilities support every stage from concept to clinic. Partner with us to accelerate your diagnostic development with reliable, quality-controlled materials.