Anti-PCIF1 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9H4Z3

Core Product Specifications and Parameters

Biological Background: PCIF1 Function and Localization

• PCIF1 (phosphorylated CTD-interacting factor 1), also known as CAPAM or hCAPAM, is a cap-specific adenosine methyltransferase encoded by the PCIF1 gene on chromosome 20.
• It catalyzes the formation of N6,2'-O-dimethyladenosine (m6Am) by methylating the adenosine at the second transcribed position of capped mRNAs. Related references: PMID:30467178 PMID:30487554 PMID:31279658
• PCIF1 is recruited to the RNA polymerase II elongation complex through interaction with the POLR2A subunit, mediating co-transcriptional m6Am modification. Related references: PMID:30467178
• It localizes to the nucleus, consistent with its role in mRNA processing.
• PCIF1 is ubiquitously expressed, suggesting a general function in mRNA cap modification across tissues.
• The protein is classified as a phosphoprotein and belongs to the methyltransferase family, indicating regulation by phosphorylation and a role in epitranscriptomic control.
• Alternative names include CAPAM (Cap-specific adenosine methyltransferase), PPP1R121 (protein phosphatase 1 regulatory subunit 121), and C20orf67, reflecting its diverse functional annotations.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 81 kDa is expected; consider denaturing and reducing conditions and verify in lysates from human, mouse, or rat cells.
• Immunohistochemistry (paraffin) may require antigen retrieval optimization; validate in target tissues with appropriate controls.
• For immunoprecipitation and ELISA, titrate antibody and confirm specificity using recombinant protein or competitive peptides if available.
• Cross-reactivity with mouse and rat allows broader model organism applications, but confirm sequence homology in the immunogen region for your specific experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, core labs, and research institutes with high-quality IVD raw materials such as validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our portfolio includes PCIF1-targeting reagents for epigenetic and RNA modification research, supporting projects from early discovery through assay development. With integrated sourcing and technical consulting, we help streamline your workflow and accelerate time-to-clinic for mRNA-based diagnostics.