Anti-Olig2 Monoclonal Antibody for WB, IF, IHC, ELISA - Q13516

Core Product Specifications and Parameters

Biological Background: Olig2 Function and Localization

• Oligodendrocyte transcription factor 2 (Olig2), also known as BHLHB1, BHLHE19, PRKCBP2, and RACK17, is a basic helix-loop-helix transcription factor encoded by the OLIG2 gene.
• Essential for oligodendrocyte and motor neuron specification in the spinal cord and hindbrain development.
• Cooperates with ZNF488 to drive oligodendrocyte differentiation and with OLIG1 to establish the pMN domain of the neural tube.
• Functions as a key regulator of progenitor cell fates during cortical gliogenesis and gliomagenesis, including activation of oligodendrocyte precursor cells (OPCs) and repression of GSX2-dependent olfactory bulb interneuron progenitors.
• Antagonizes V2 interneuron development and NKX2-2-induced V3 interneuron differentiation.
• Subcellular localization includes the nucleus and cytoplasm, consistent with its role as a DNA-binding transcription factor.
• Highly expressed in oligodendrocytes and overexpressed in oligodendrogliomas; expression variable in glioblastomas; also linked to chromosomal rearrangements in certain cancers.
• Keywords: chromosomal rearrangement, proto-oncogene, developmental protein, transcription regulation, DNA-binding, nucleus, cytoplasm, Ubl conjugation.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide corresponding to human Olig2 and is recommended for Western blot (WB), immunofluorescence (IF-P), immunohistochemistry (IHC-P), and ELISA.
• Given the nuclear and cytoplasmic localization of Olig2, ensure thorough sample lysis and consider nuclear fractionation for stronger signal in WB.
• For immunohistochemistry, test antigen retrieval methods to optimize detection in formalin-fixed paraffin-embedded tissues.
• Validate the antibody in the relevant species (human, mouse, rat) using appropriate positive and negative controls, such as oligodendrocyte-rich brain tissues or cell lines.
• Since Olig2 is a transcription factor, its expression levels may be moderate; dilute the antibody according to the recommended starting range and adjust based on observed signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This Olig2 antibody supports studies in neurobiology, gliogenesis, and cancer research. For custom sourcing or rare target reagents, CamelBio offers tailored procurement and technical consulting services to accelerate your assay development from concept to clinic.