Anti-MMUT Monoclonal Antibody for WB, IHC-P, ELISA - P22033

Core Product Specifications and Parameters

Biological Background: Methylmalonyl-CoA Mutase (MMUT) Function and Localization

• Methylmalonyl-CoA mutase (MMUT) is a mitochondrial enzyme that catalyzes the reversible isomerization of methylmalonyl-CoA to succinyl-CoA, a crucial step in the metabolism of propionate derived from branched-chain amino acids, odd-chain fatty acids, and cholesterol.
• The enzyme requires adenosylcobalamin (a derivative of vitamin B12) as a cofactor and binds cobalt, highlighting its dependence on cobalamin for catalytic activity.
• MMUT functions predominantly in the mitochondrial matrix, with additional detection in the cytoplasm, consistent with its role in mitochondrial energy metabolism.
• Post-translational modifications of MMUT include acetylation and phosphorylation, indicating regulatory mechanisms that may modulate its activity.
• Mutations in the MMUT gene are associated with methylmalonic aciduria, an inborn error of metabolism characterized by accumulation of methylmalonic acid.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 83 kDa is anticipated, corresponding to the full-length protein. Consider using mitochondrial enrichment to enhance detection.
• In immunohistochemistry, due to the mitochondrial localization, ensure appropriate antigen retrieval methods are employed to expose the epitope.
• When applying in ELISA, pairing with another antibody targeting a distinct epitope may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering a wide range of IVD raw materials including validated antibody pairs, monoclonal antibodies, and ancillary reagents. For researchers focusing on metabolic disorders and mitochondrial function, CamelBio can source MMUT-related reagents and support assay development from concept to clinic.