Anti-MARS Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P56192

Core Product Specifications and Parameters

Biological Background: MARS Function and Localization

• Encodes methionine--tRNA ligase, cytoplasmic (MetRS), which catalyzes the specific attachment of L-methionine to its cognate tRNA in a two-step reaction: amino acid activation by ATP and transfer to tRNA. Related references: PMID:11714285, PMID:33909043
• Acts as an editing enzyme preventing homocysteine misincorporation by hydrolyzing misactivated homocysteinyl-AMP to homocysteine-thiolactone and AMP.
• Plays a role in ribosomal RNA synthesis within the nucleolus. Related references: PMID:10791971
• Localizes to the cytosol and nucleus, with enrichment in the nucleolus.
• Contains ATP-, RNA-, and tRNA-binding domains, characteristic of class I aminoacyl-tRNA synthetases.
• Phosphoprotein implicated in protein biosynthesis and subject to alternative splicing.
• Associated with neurological diseases including Charcot-Marie-Tooth disease and hereditary spastic paraplegia; mutations linked to clinical variants.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–270, covering the N-terminal region; consider that the resulting polyclonal antibody may recognize multiple epitopes and detect isoforms or fragments.
• For WB, the predicted molecular weight is ~101 kDa; validate detection in appropriate positive control lysates (e.g., human or rat cell lines) and optimize blocking/dilution conditions.
• For IHC-P, antigen retrieval conditions and antibody dilution should be empirically optimized for each tissue type; include appropriate controls.
• For ELISA, pair with a suitable detection antibody and validate specificity using recombinant antigen or lysates.
• Because of the protein’s role in translation and neurological disorders, this antibody may be useful in translational research; ensure validation in the relevant disease model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated IVD raw materials—including monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents—to diagnostic manufacturers and researchers worldwide. This anti-MARS antibody supports studies in aminoacyl-tRNA synthetase biology, protein synthesis, and neurological disease mechanisms. Leverage our sourcing expertise to accelerate your assay development from concept to clinic.