Anti-LIG1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P18858

Core Product Specifications and Parameters

Biological Background: LIG1 Function and Localization

• DNA ligase 1 (LIG1) is an ATP-dependent enzyme that seals nicks in double-stranded DNA by catalyzing the formation of phosphodiester bonds.
• It plays a critical role in DNA repair, sealing nicks generated during excision repair and other pathways. Related references: PMID:30395541
• LIG1 is also involved in DNA replication, where it joins Okazaki fragments on the lagging strand, and participates in DNA recombination.
• The protein localizes to the nucleus and is active throughout the cell cycle, with increased expression during cell division.
• Key post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interactions.
• The predicted molecular weight of LIG1 is approximately 102 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 565-790) of human LIG1, suggesting the antibody may recognize both full-length and C-terminal isoforms, if present.
• For Western blotting, expect a specific band near 102 kDa. Validate the antibody in appropriate positive controls such as HeLa cell lysate or mouse tissue extracts.
• When performing immunofluorescence/ICC, confirm the expected nuclear staining pattern, consistent with LIG1’s localization.
• Optimize antibody dilution for each application and sample type; typical starting dilutions can be determined empirically.
• Cross-reactivity is reported for human and mouse; verify reactivity in other species before use.
• This antibody is suitable for ELISA; consider pairing with a matched detection antibody for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. This LIG1 antibody enables studies in DNA repair, replication, and genomic stability, making it useful for diagnostic assay development in these areas. Beyond this polyclonal antibody, CamelBio offers a comprehensive range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets, ensuring a reliable supply chain for your diagnostic needs.