Anti-IDH1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O75874

Core Product Specifications and Parameters

Biological Background: IDH1 Function and Localization

• Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1), also known as cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, and oxalosuccinate decarboxylase.
• Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate, which is required by other enzymes such as phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. Related references: PMID:10521434 PMID:19935646
• Subcellular localization includes cytoplasm, cytosol, and peroxisome.
• May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.
• Post-translational modifications include acetylation and phosphorylation; the protein binds magnesium or manganese as cofactors and uses NADP.
• Part of the tricarboxylic acid cycle and glyoxylate bypass; encoded by the IDH1 gene (also known as PICD).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human IDH1. This region is conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• For Western blotting (WB), the expected molecular weight is approximately 47 kDa. Validate detection in relevant cell or tissue lysates from human, mouse, or rat.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect cytoplasmic and peroxisomal staining patterns. Use appropriate fixation and permeabilization conditions for optimal signal.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pair with another antibody recognizing a distinct epitope for sandwich assays.
• Specificity can be confirmed by pre-adsorption with the immunizing peptide or by using IDH1 knockout cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials and technical services covering every stage from concept to clinic. For targets like IDH1—relevant to cancer metabolism, neurodegenerative diseases, and redox biology—we offer validated monoclonal/polyclonal antibodies, optimized antibody pairs for immunoassays, and bulk ancillary reagents. Contact us to streamline your sourcing of rare-target raw materials and accelerate your assay development.