Anti-IAH1 Rabbit Polyclonal Antibody for WB, ELISA - Q2TAA2

Core Product Specifications and Parameters

Biological Background: IAH1 Function and Localization

• IAH1 encodes isoamyl acetate-hydrolyzing esterase 1 homolog, a member of the hydrolase family of enzymes.
• The protein is annotated as a probable lipase, indicating a potential role in the breakdown of lipids.
• Alternative splicing of the IAH1 gene may generate multiple transcript variants, though specific isoforms are not yet characterized.
• UniProt keywords include lipid degradation and lipid metabolism, placing IAH1 in pathways responsible for lipid catabolism.
• The protein has been identified through proteomics, confirming its expression at the protein level in the human reference proteome.
• The predicted molecular weight of the reference protein is approximately 27.6 kDa, while the observed band migrates at around 28 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (amino acids 1–248) of human IAH1, which is likely present in all potential splice variants; thus, the antibody may recognize multiple isoforms.
• For Western blot, expect a band near 28 kDa; low molecular weight proteins may require careful optimization of transfer conditions and blocking steps.
• Cross-reactivity with mouse and rat suggests conserved epitopes; validation in the specific species and tissue of interest is recommended.
• In ELISA applications, establish optimal antibody coating concentrations and detector conditions using purified IAH1 protein or cell lysates as a reference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, supporting diagnostic manufacturers and researchers from concept to clinic. For lipid metabolism and hydrolase-targeted projects, we offer validated polyclonal antibodies like IAH1 Rabbit pAb, along with bulk ancillary reagents and rare-target sourcing services. Our integrated supply chain and technical consultation ensure reliable access to the materials you need for assay development and validation.