Anti-HYAL1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12794

Core Product Specifications and Parameters

Biological Background: HYAL1 Function and Localization

• HYAL1 (Hyaluronidase-1) is a lysosomal and secreted hydrolase that degrades hyaluronic acid, a major component of the extracellular matrix.
• It belongs to the hyaluronidase family and contains an EGF-like domain and disulfide bonds critical for its structure and function.
• HYAL1 is highly expressed in liver, kidney, and heart, with lower expression in lung, placenta, and skeletal muscle; it is absent in adult brain.
• Multiple isoforms exist due to alternative splicing; for example, isoform 1 is restricted to bladder and prostate cancer cells, while isoforms 3-6 are found in normal and tumor bladder tissues.
• The protein may promote tumor progression by degrading extracellular matrix hyaluronan, facilitating invasion and metastasis.
• It may also modulate cell growth by blocking TGFB1-enhanced proliferation.
• Subcellularly, HYAL1 localizes to lysosomes and is also secreted into the extracellular milieu.
• Genetic variants in HYAL1 are associated with mucopolysaccharidosis type IX, a lysosomal storage disorder.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 48 kDa; consider validating the detected band using appropriate positive controls and lysates from HYAL1-expressing tissues (e.g., liver, kidney).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval conditions may need optimization. Test both enzymatic and heat-mediated methods on your specific tissue or cell samples.
• The antibody shows cross-reactivity with mouse and rat; we recommend verifying specificity and sensitivity in the relevant species model before large-scale experiments.
• Given the polyclonal nature and the immunogen spanning a large region (aa 136-435), it may recognize multiple HYAL1 isoforms; confirm band patterns against known isoform profiles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and research labs. This anti-HYAL1 rabbit polyclonal antibody supports cancer biology and lysosomal storage disorder studies. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare-target proteins, helping you accelerate assay development from concept to clinic.