Anti-HNRNPR Rabbit Polyclonal Antibody [KO Validated] for WB, IF/ICC, ELISA - O43390

Core Product Specifications and Parameters

Biological Background: HNRNPR Function and Localization

• HNRNPR (heterogeneous nuclear ribonucleoprotein R) is a member of the hnRNP family, which comprises at least 20 different RNA-binding proteins that assemble into ribonucleosome complexes.
• As part of these complexes, it participates in precursor mRNA (pre-mRNA) processing in the nucleus, particularly in splicing and other post-transcriptional modifications.
• The protein is an integral component of the spliceosome, a large ribonucleoprotein complex responsible for pre-mRNA splicing, and influences alternative splicing decisions.
• HNRNPR localizes mainly to the nucleus, particularly the nucleoplasm, but can also be detected in the cytoplasm and associated with microsomal/endoplasmic reticulum (ER) membranes.
• Post-translational modifications include acetylation and ISGylation (a type of ubiquitin-like modification), which may regulate protein stability, interactions, or subcellular distribution.
• Mutations in the HNRNPR gene have been associated with intellectual disability and other neurological disease phenotypes (keyword: Disease variant, Intellectual disability).
• The protein has a predicted molecular weight of approximately 71 kDa and is evolutionarily conserved among mammals, including human, mouse, and rat.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 550-633) of human HNRNPR; this sequence is highly conserved in mouse and rat, supporting the reported cross-reactivity.
• For Western blotting, a band near 71 kDa is expected; use standard nuclear or whole-cell lysates from human, mouse, or rat cell lines (e.g., HeLa, NIH/3T3) as positive controls.
• For immunofluorescence, expect predominantly nuclear staining with possible faint cytoplasmic signal; optimize fixation/permeabilization conditions and validate with a knockdown or knockout model if available.
• ELISA performance may vary; consider optimizing the antibody dilution and using a recombinant HNRNPR protein as a standard for quantitation.
• If adapting this antibody for immunoprecipitation or chromatin immunoprecipitation (ChIP), perform rigorous validation with appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop supplier of IVD raw materials, dedicated to bridging the gap from concept to clinic for diagnostic manufacturers and research laboratories worldwide. For your HNRNPR-related projects in pre-mRNA processing and genetic neurological disease research, we offer validated antibody pairs, optimized recombinant monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expert team can also assist in sourcing rare-target raw materials and providing tailored technical support to accelerate your assay development.