Anti-HNRNPD Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q14103

Core Product Specifications and Parameters

Biological Background: HNRNPD Function and Localization

• HNRNPD, also known as AUF1, is a heterogeneous nuclear ribonucleoprotein that binds AU-rich elements (AREs) in the 3'-UTR of mRNAs, regulating mRNA stability and turnover.
• It functions as a transcription factor by binding to double- and single-stranded DNA sequences, including telomeric repeats, and may inhibit DNA quadruplex formation to regulate telomere elongation.
• HNRNPD is involved in the post-transcriptional regulation of proto-oncogenes and cytokines, playing a critical role in cancer, inflammation, and immune response.
• It participates in the rhythmic expression of circadian clock genes by directly binding to the 3'UTR of CRY1 mRNA and enhancing its translation, and may also regulate PER2 translation.
• The protein localizes to both the nucleus and cytoplasm, shuttling between these compartments and undergoing various post-translational modifications such as phosphorylation, acetylation, methylation, and ubiquitination.
• Alternative splicing of the HNRNPD gene produces multiple isoforms (e.g., P37, AUF1A) that may have distinct functions and expression patterns.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-306 of human HNRNPD, which corresponds to the full-length canonical isoform (NP_002129.2). This covers all RNA recognition motifs (RRMs) and functional domains, making it suitable for detecting multiple isoforms across species.
• Based on the specified applications, this antibody can be used for Western blot (predicted band at ~38 kDa), immunoprecipitation, ELISA, and immunoassays like IHC-P and IF/ICC. End-users should optimize dilutions and validate performance in their specific sample systems.
• Given the high sequence similarity among human, mouse, and rat HNRNPD (as indicated by cross-reactivity), this antibody is a valuable tool for comparative studies in these species. However, species-specific validation is recommended for other organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of high-quality IVD antibodies and ancillary reagents, including validated polyclonal antibodies like this HNRNPD target for transcriptional and post-transcriptional regulation research. With one-stop sourcing from concept to clinic, we support diagnostic manufacturers and laboratories in accelerating assay development and securing raw materials for rare and common targets. Explore our selection of optimized antibody pairs, bulk antibodies, and customized procurement solutions for your next project.