Anti-hnRNP C Rabbit Monoclonal Antibody for WB, IF, ELISA - P07910

Core Product Specifications and Parameters

Biological Background: hnRNP C Function and Localization

• HNRNPC encodes heterogeneous nuclear ribonucleoproteins C1 and C2 (hnRNP C1/C2), which are core RNA-binding components of hnRNP particles.
• hnRNP C binds pre-mRNA and nucleates the assembly of 40S hnRNP particles, a critical step in mRNA maturation (PubMed:8264621). Related references: PMID:8264621
• It interacts with poly-U tracts in 3'-UTRs and 5'-UTRs, modulating mRNA stability and translation (PubMed:12509468, PubMed:16010978, PubMed:7567451). Related references: PMID:12509468 | PMID:16010978 | PMID:7567451
• Single HNRNPC tetramers bind approximately 230–240 nucleotides; trimers of tetramers span up to 700 nucleotides, facilitating RNP coating (PubMed:8264621). Related references: PMID:8264621
• The protein participates in early spliceosome assembly and pre-mRNA splicing; N6-methyladenosine (m6A) modifications can induce local RNA structural alterations (m6A-switch), enhancing HNRNPC binding and regulating alternative splicing (PubMed:25719671). Related references: PMID:25719671
• hnRNP C localizes exclusively to the nucleus, consistent with its role in RNA processing and ribonucleoprotein complex formation.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitination; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises residues 80–170 of human hnRNP C, a conserved RNA-binding domain region; verify sequence identity in mouse to ensure robust cross-reactivity.
• For Western blot (WB), hnRNP C isoforms C1/C2 migrate at ~34–36 kDa; use appropriate positive controls such as HeLa or HEK293 nuclear extracts.
• In immunofluorescence (IF/ICC, IF-P), anticipate nuclear staining; permeabilization and fixation conditions should be optimized for nuclear antigens.
• For ELISA, the recombinant immunogen fragment can serve as a positive control to assess antibody binding.
• Consider validating the antibody in your specific sample system, as cross-reactivity has been confirmed for human and mouse but not other species.

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CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. For RNA processing and splicing studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-hnRNP C clone, along with bulk ancillary reagents and rare-target sourcing. Simplify your procurement through our integrated supply chain solutions.