Anti-hnRNP A1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09651

Core Product Specifications and Parameters

Biological Background: hnRNP A1 Function and Localization

• Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), also known as UP1, ALS19, ALS20, or HNRPA1, is a multifunctional RNA-binding protein encoded by the HNRNPA1 gene in humans.
• Core role in mRNA metabolism: packages pre-mRNA into hnRNP particles, mediates nucleocytoplasmic transport of poly(A) mRNA, and modulates splice site selection, thereby influencing alternative splicing decisions. Related references: PMID:17371836
• Regulates alternative splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and promoting PKM M2 isoform production. Related references: PMID:20010808
• Contributes to translational control by binding to the internal ribosome entry site (IRES) of APAF1 mRNA, thereby inhibiting translation of the apoptosis protease activating factor APAF1. Related references: PMID:31498791
• May interact with specific miRNA hairpins, expanding its regulatory potential in post-transcriptional gene silencing. Related references: PMID:28431233
• Shuttles between nucleus and cytoplasm; predominantly nuclear but localized to the cytoplasm under certain conditions, consistent with its roles in RNA processing and transport.
• Subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitin-like conjugation, as suggested by proteomic studies.
• Implicated in neurodegenerative diseases: mutations in HNRNPA1 are associated with amyotrophic lateral sclerosis (ALS) and inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering full-length human hnRNP A1 (amino acids 1–372). This broad immunogen may enable recognition of multiple isoforms or splice variants; performance should be validated in the relevant experimental context.
• For Western blotting, a band corresponding to the predicted molecular weight of ~39 kDa is expected. Consider using whole cell lysates from human, mouse, or rat tissues/cells, as cross-reactivity is confirmed in these species.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimise antigen retrieval and antibody dilution for the specific tissue or cell type, as localisation may be predominantly nuclear or cytoplasmic depending on cellular state.
• When performing ELISA, ensure that the coating antigen maintains the native conformation or key epitopes present in the immunogen to maximise detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated antibodies, recombinant proteins, and ancillary reagents. For research focused on RNA processing, neurodegenerative diseases, or cancer metabolism, we supply critical reagents such as anti-hnRNP A1 polyclonal antibodies, along with the capability to source monoclonal alternatives, matched antibody pairs, and bulk quantities to support assay development and scale-up. Whether you require standard catalogue items or rare-target material procurement, our technical and supply chain expertise helps streamline your workflow from concept to clinic.