Anti-HABP4 Rabbit Polyclonal Antibody for WB, ELISA - Q5JVS0

Core Product Specifications and Parameters

Biological Background: HABP4 Function and Localization

• HABP4, also known as intracellular hyaluronan-binding protein 4 or Ki-1/57 intracellular antigen, is a ribosome-binding protein that promotes ribosome hibernation, stabilizing inactive ribosomes and protecting them from proteasomal degradation (By similarity).
• It binds RNA, regulating transcription and pre-mRNA splicing, and specifically binds to poly(U) RNA via its C-terminus. Related references: PMID:14699138, PMID:16455055, PMID:19523114
• HABP4 appears to play a role in the formation of PML nuclear bodies. Related references: PMID:28695742
• It negatively regulates the DNA-binding activity of the transcription factor MEF2C in myocardial cells in response to mechanical stress (By similarity).
• Subcellularly, HABP4 localizes to the nucleus, cytoplasm, stress granules, sarcoplasm, and various nuclear bodies including nucleolus, speckles, Cajal bodies, and gems.
• Tissue expression is highest in brain, heart, and kidney, with moderate expression in skeletal muscle; it is also found in tumor cell lines and activated leukocytes.
• Post-translational modifications include phosphorylation and methylation; it also undergoes ubiquitination (isopeptide bond and Ubl conjugation).
• Alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide encompassing residues 50–150; thus reactivity is expected against denatured protein, making it suitable for Western blot under reducing conditions.
• For WB, the predicted molecular weight is ~46 kDa; confirm specificity by including appropriate positive (e.g., HeLa, HEK293) and negative controls.
• Cross-reactivity with mouse and rat is reported; validate in the target species’ tissue or cell lysate to ensure equivalent performance.
• For ELISA applications, optimize coating buffer, antibody titer, and detection system empirically to achieve the required dynamic range.
• As a polyclonal antibody, lot-to-lot consistency should be verified for reproducibility in quantitative assays.
• Upon receipt, store the antibody according to the manufacturer’s guidelines; the product is shipped on ice to preserve stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop source for IVD raw materials, technical support, and consulting from discovery through commercialization. For projects targeting HABP4 in transcriptional regulation, ribosome biology, or cancer biomarker development, we supply validated antibodies like this HABP4 Rabbit pAb, along with matched antibody pairs, recombinant proteins, and bulk ancillary reagents. Our sourcing capabilities also extend to hard-to-find target raw materials, streamlining your assay development pipeline.