Anti-GIPC1 Rabbit Polyclonal Antibody for WB, ELISA - O14908

Core Product Specifications and Parameters

Biological Background: GIPC1 Function and Localization

• GIPC1 (PDZ domain-containing protein GIPC1) is also known as GAIP C-terminus-interacting protein, synectin, and RGS19-interacting protein 1, and contains a PDZ domain that mediates protein-protein interactions.
• It may play a role in G protein-linked signaling pathways, interacting with regulators of G-protein signaling (RGS) proteins.
• The protein is widely expressed in various tissues, as demonstrated by mRNA expression analysis, and is also detected at the protein level in skeletal muscle. Related references: PMID:9770488 PMID:32413282
• GIPC1 localizes primarily to the cytoplasm and membrane, consistent with its role in membrane-associated signaling complexes.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, potentially generating multiple isoforms.
• Mutations or dysregulation of GIPC1 have been associated with congenital muscular dystrophy and dystroglycanopathy, highlighting its importance in muscle function.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of GIPC1 is approximately 36 kDa; however, actual migration may vary depending on gel conditions and post-translational modifications—validate using positive control lysates from human, mouse, or rat tissues.
• Given the immunogen is a full-length recombinant fusion protein (aa 1-333), the antibody may recognize multiple isoforms; confirm specificity in your experimental system.
• For ELISA applications, titrate the antibody and optimize coating and blocking conditions for best signal-to-noise ratio.
• Based on expression profiles, skeletal muscle lysates may serve as a suitable positive control for Western blot; consider validating in your own sample panel.

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