Anti-FOXP3 Monoclonal Antibody for WB, IHC-P, ELISA - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 is a forkhead box transcription factor and master regulator essential for the development and suppressive function of regulatory T-cells (Treg). It acts as a transcriptional activator or repressor depending on cofactor interactions, controlling immune homeostasis. Related references: PMID:17377532 PMID:21458306 PMID:23947341
• Deeply involved in maintaining peripheral tolerance, FOXP3 prevents autoimmunity by repressing pro-inflammatory cytokines such as IL-2 and IFN-γ, while activating CTLA4 and TNFRSF18, directly modulating conventional T-cell expansion. Related references: PMID:23169781
• Mechanistically, FOXP3 inhibits IL-2 transcription through association with histone acetylase KAT5 and histone deacetylase HDAC7, and can suppress RELA and NFATC2 activity to block T-cell effector functions. Related references: PMID:17360565 PMID:15790681
• FOXP3 antagonizes RORC to inhibit Th17 cell differentiation and downregulates IL-17 expression, thereby favoring Treg lineage commitment. Related references: PMID:18368049
• It also directly represses the transcriptional activity of RORA, further influencing immune cell fate. Related references: PMID:18354202
• Subcellular localization includes both nucleus and cytoplasm, with nuclear import essential for its transcriptional regulatory functions.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination dynamically modulate FOXP3 stability and activity.
• Mutations in FOXP3 cause IPEX syndrome (immune dysregulation, polyendocrinopathy, enteropathy, X-linked), highlighting its critical role in immune tolerance.

Experimental Guidance and Technical Tips

• For Western blot analysis, a specific band around 47 kDa is expected under reducing conditions. Validate with appropriate positive controls such as Treg cell lysates.
• In immunohistochemistry (paraffin-embedded tissues), strong nuclear staining with possible cytoplasmic signal is typically observed in CD4+ regulatory T-cells. Optimize antigen retrieval and detection reagents for your experimental setup.
• This recombinant protein-derived monoclonal antibody may be suitable for ELISA-based assays, but performance should be verified in the relevant system and matrix.
• As cross-reactivity has been reported for human, mouse, and rat, allelic variation across these species may affect binding affinity; consider sequence alignment when designing multi-species experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing support. For FOXP3, a key Treg marker critical in immunology and autoimmune disease research, we offer reliable raw materials to accelerate your assay development from concept to clinic. Contact our team to discuss your specific procurement needs.