Anti-DNASE1L1 Polyclonal Antibody for WB, IF/ICC, ELISA - P49184

Core Product Specifications and Parameters

Biological Background: DNASE1L1 Function and Localization

• DNASE1L1, also designated DNase X, DNase I-like 1, and XIB, is a member of the deoxyribonuclease I (DNase I) family.
• The protein bears a signal peptide for secretion, a conserved disulfide bond, and N-linked glycosylation sites.
• Subcellular localization is primarily the endoplasmic reticulum.
• Tissue expression is highest in skeletal and cardiac muscles, with lower levels in most other tissues, except brain.
• Based on sequence homology and family assignment, DNASE1L1 is predicted to function as an endonuclease, catalyzing DNA hydrolysis.
• The calculated molecular weight is approximately 33.9 kDa, consistent with the observed 34 kDa band.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human DNASE1L1 sequence (aa 1–302); this antibody may recognize endogenous, processed, or variant forms of the protein.
• For Western blotting, a band around 34 kDa is expected. Consider using skeletal or cardiac muscle lysates as positive controls, given the highest expression in these tissues.
• In immunofluorescence and immunocytochemistry, expect a reticular staining pattern corresponding to the endoplasmic reticulum.
• Reported cross-reactivity with mouse and rat is denoted; validation in the target species’ sample matrix is advised before quantitative interpretation.
• ELISA performance may require optimization of coating antigen and antibody titer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This high-quality anti-DNASE1L1 polyclonal antibody complements our portfolio of validated antibodies, recombinant proteins, and ancillary reagents for nuclease-targeted assay development. Whether you need optimized antibody pairs, bulk monoclonal/polyclonal antibodies, or assistance with rare-target sourcing, CamelBio streamlines your procurement to accelerate diagnostics development.