Anti-DNA topoisomerase I (TOP1) Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P11387

Core Product Specifications and Parameters

Biological Background: DNA topoisomerase I Function and Localization

• DNA topoisomerase 1 (TOP1) is a monomeric enzyme encoded by the TOP1 gene (GeneID: 7150). It is also known as DNA topoisomerase I and is classified as a type IB topoisomerase.
• TOP1 resolves DNA supercoiling and torsional tension generated during replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex, allowing controlled rotation of the broken strand around the intact strand (By similarity).
• TOP1 forms a covalent DNA-(3′-phosphotyrosyl)-enzyme intermediate through transesterification, and then religates the DNA after supercoil removal; this activity is essential for DNA metabolism.
• Beyond its canonical role, TOP1 regulates alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and is involved in circadian transcription by modulating chromatin structure at the BMAL1 promoter.
• The protein localizes to the nucleus, specifically the nucleolus and nucleoplasm, consistent with its roles in DNA transcription and replication.
• TOP1 undergoes various post-translational modifications including acetylation and phosphorylation, and is a substrate for ubiquitin-like conjugation.
• It is expressed in endothelial cells and has been implicated in chromosomal rearrangements and host-virus interactions, highlighting its broader biological and clinical significance.
• The calculated molecular weight is approximately 91 kDa, which aligns with the observed band in western blotting.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal peptide (aa 1–100); therefore, it is expected to recognize full-length TOP1 and may also detect N-terminal variants. Verify specificity in the relevant experimental system.
• TOP1 is predominantly nuclear; effective nuclear extraction and fixation/permeabilization protocols (e.g., Triton X-100 treatment) are recommended for IHC-P and IF/ICC.
• For WB, use reducing conditions; the predicted band appears near 91 kDa. Include appropriate positive controls such as HeLa or endothelial cell lysates.
• This rabbit monoclonal offers high specificity and low background; however, optimal antibody dilutions should be determined empirically for each application and sample type.
• IP and ELISA protocols may require optimization of antibody concentration and incubation conditions; consider cross-linking the antibody to resin for IP.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-TOP1 monoclonal antibody supports research in DNA topology, cancer biology, and chromatin dynamics. For your IVD development needs, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in rare target raw-material sourcing.