Anti-DNA Polymerase gamma Monoclonal Antibody for WB, ELISA - P54098

Core Product Specifications and Parameters

Biological Background: DNA Polymerase gamma Function and Localization

• DNA polymerase subunit gamma-1 (POLG) is the catalytic core of mitochondrial DNA polymerase, responsible for the replication of the entire mitochondrial genome. It synthesizes both heavy and light strands using single-stranded DNA templates and RNA primers, functioning as a highly processive replisome together with TWNK helicase and SSBP1. Related references: PMID:11477093, PMID:11897778, PMID:15917273
• POLG possesses a 3'→5' exonuclease proofreading activity that corrects base substitutions, single-base additions, and deletions, ensuring high fidelity during mtDNA replication. When a misincorporation occurs, the enzyme transitions into a nucleolytic editing mode to remove the erroneous nucleotide. Related references: PMID:10827171, PMID:11477094, PMID:11504725
• After completing mtDNA synthesis, POLG mediates 3'→5' exonucleolytic degradation at the nick to facilitate proper ligation, and it also exhibits 5'-deoxyribose phosphate (dRP) lyase activity, enabling its participation in the mitochondrial base-excision repair pathway. Related references: PMID:9770471, PMID:11477093, PMID:15167897
• Mutations in the POLG gene are linked to a spectrum of mitochondrial disorders, including progressive external ophthalmoplegia (PEO), neuropathies, epilepsy, and Leigh syndrome. It is a primary mitochondrial disease gene associated with mitochondrial DNA depletion and deletion syndromes.
• The protein localizes to the mitochondrial matrix and is a component of the mitochondrial nucleoid, where it associates with mtDNA to facilitate replication and maintenance.
• POLG is a large, multi-functional enzyme (approximately 140 kDa) that belongs to the DNA polymerase type-A family. It requires magnesium ions for polymerase and exonuclease activities.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to a peptide within the C-terminal region (amino acids 1140–1239) of human POLG. This epitope lies outside the catalytic domains and may provide recognition of both wild-type and some mutant forms. However, for each experimental system, verify epitope accessibility under native and denaturing conditions.
• For Western blot, the predicted molecular weight is 140 kDa. Use appropriate gel percentage and transfer conditions for high molecular weight proteins, and include a positive control such as mitochondrial lysates from human or mouse cells.
• Cross-reactivity with mouse POLG has been confirmed, making this antibody suitable for studies in mouse models of mitochondrial disease. Due to high sequence conservation, cross-reactivity with other species may be possible but should be empirically validated.
• For ELISA, the antibody can be used as a capture or detection reagent. Titrate the antibody to determine the optimal working concentration, and ensure the coating antigen contains the C-terminal epitope or a recombinant POLG fragment spanning the immunogen region.
• Note that POLG expression is ubiquitous but enriched in tissues with high energy demand. Consider validating detection sensitivity in relevant sample systems, such as liver, muscle, or brain mitochondrial preparations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers and researchers, offering ready access to validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom rare-target raw material sourcing. For studies involving mitochondrial DNA replication and repair, we provide this high-quality anti-POLG antibody alongside complementary reagents to accelerate your diagnostic development. Our integrated supply model supports every stage from concept to clinic, helping you reduce sourcing complexity and lead times.