Anti-DNA polymerase eta Polyclonal Antibody for WB - Q9Y253

Core Product Specifications and Parameters

Biological Background: DNA polymerase eta Function and Localization

• DNA polymerase eta (POLH), also known as RAD30A or XP-V protein, is a Y-family DNA polymerase specialized in translesion synthesis (TLS) across UV-induced cyclobutane pyrimidine dimers and other distorting lesions. Related references: PMID:10385124, PMID:11743006, PMID:16357261
• It cooperates with the POLZ complex (REV3L-REV7-POLD2-POLD3) to extend primers after lesion bypass, inserting one or two nucleotides opposite the damaged site. Related references: PMID:24449906
• POLH targets DNA polymerase iota (POLI) to replication foci, facilitating a coordinated damage response. Related references: PMID:12606586
• At abasic sites, POLH forms a Schiff base with 5′-deoxyribose phosphate, covalently trapping the enzyme and inhibiting its DNA synthesis to prevent mutagenesis during base excision repair. Related references: PMID:14630940
• The protein localizes to the nucleus and is enriched at replication foci upon DNA damage.
• POLH is subject to ubiquitination, forming isopeptide bonds that modulate its recruitment and activity.
• Mutations in POLH are responsible for Xeroderma pigmentosum variant (XP-V), a recessive disorder marked by extreme UV sensitivity and a high risk of skin cancer.
• It also plays a role in somatic hypermutation of immunoglobulin genes, contributing to antibody diversity. Related references: PMID:11376341, PMID:14734526

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region (aa 322–520) of human POLH, which contains conserved domains critical for polymerase activity and lesion bypass.
• For Western blot (WB), expect a specific band at approximately 78 kDa in nuclear lysates from human, mouse, or rat cell lines.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization to detect nuclear foci, which increase after UV treatment.
• For ELISA, pre-coat the immunogen protein or use a direct coating approach, and consider a blocking step to minimize background.
• Validate antibody specificity in knockout or siRNA-treated samples to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD developers and research teams by supplying high-quality raw materials for DNA damage and repair studies. This anti-POLH polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, recombinant proteins, and ancillary reagents. Leverage CamelBio’s integrated sourcing and technical consultation to accelerate assay development from discovery to clinical application.