Anti-DNA Ligase IV Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49917

Core Product Specifications and Parameters

Biological Background: DNA Ligase IV Function and Localization

• DNA ligase 4 (LIG4), also known as DNA ligase IV or polydeoxyribonucleotide synthase [ATP] 4, is encoded by the LIG4 gene and is a member of the ATP-dependent DNA ligase family.
• LIG4 catalyzes the final ligation step in non-homologous end joining (NHEJ), an essential pathway for repairing DNA double-strand breaks (DSBs) and for V(D)J recombination during immune development. Related references: PMID:12517771, PMID:17290226, PMID:23523427, PMID:9242410
• LIG4 forms a tight subcomplex with the scaffold protein XRCC4, which markedly stimulates its ligation activity; the LIG4-XRCC4 complex can seal single-strand nicks and, with lower efficiency, join ends bearing 2-nucleotide microhomology or 1-nucleotide gaps. Related references: PMID:17290226, PMID:9242410, PMID:9809069
• Efficient loading of the LIG4-XRCC4 complex onto DNA breaks requires the assembly of the DNA-dependent protein kinase (DNA-PK) holoenzyme at the damage site. Related references: PMID:10854421
• LIG4 also regulates the nuclear accumulation of XRCC4, underscoring a dynamic interplay that localizes the NHEJ machinery to sites of DNA damage. Related references: PMID:24984242
• The protein is predominantly nuclear, consistent with its function in genomic DNA repair, and is expressed at highest levels in testis, thymus, prostate, and heart.
• With a predicted molecular mass of ~104 kDa, LIG4 contains conserved motifs for ATP binding, magnesium/metal coordination, and tandem BRCT repeats that mediate protein–protein interactions in DNA repair complexes.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fragment spanning amino acids 1–210 of human LIG4 (NP_002303.2); when designing experiments, consider that this N‑terminal region may be conserved across species, supporting the observed cross‑reactivity.
• Validated applications include western blotting (WB), immunohistochemistry on paraffin‑embedded sections (IHC-P), and ELISA; for WB, the predicted molecular weight is approximately 104 kDa—always validate detection in the relevant cell or tissue lysate.
• Cross‑reactivity is reported for human, mouse, and rat; for experiments in other species, evaluate sequence homology of the immunogen region and include appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. This anti‑DNA Ligase IV antibody addresses the growing demand for DNA repair‑focused raw materials, supporting applications from fundamental NHEJ research to potential clinical assay design. For rare or complex targets like LIG4, CamelBio leverages an extensive network to secure high‑quality raw materials and provide seamless procurement support.