Anti-DNA Ligase IV Rabbit Monoclonal Antibody for WB, ELISA - P49917

Core Product Specifications and Parameters

Biological Background: DNA Ligase IV Function and Localization

• DNA ligase 4 (LIG4), also known as DNA ligase IV, is an ATP-dependent DNA ligase essential for non-homologous end joining (NHEJ) repair of DNA double-strand breaks (DSBs) and V(D)J recombination. It reseals broken DNA ends after gap filling. (PMID:12517771, PMID:17290226, PMID:9242410) Related references: PMID:12517771 PMID:17290226 PMID:9242410
• LIG4 forms a complex with XRCC4, which stimulates its ligation activity and enables repair of diverse DNA end configurations, including nicks, compatible overhangs, and gaps with microhomology. (PMID:17290226, PMID:9242410, PMID:9809069) Related references: PMID:17290226 PMID:9242410 PMID:9809069
• Ligation by the LIG4-XRCC4 complex is dependent on the DNA-PK complex for binding to DNA ends, coordinating NHEJ repair. (PMID:10854421) Related references: PMID:10854421
• LIG4 is localized exclusively in the nucleus, consistent with its role in genomic maintenance.
• Tissue expression of LIG4 is highest in testis, thymus, prostate, and heart, corresponding to tissues with active recombination and repair processes.
• The protein belongs to the DNA ligase family and contains conserved domains for ATP-binding and metal coordination (magnesium), critical for its catalytic function.
• Mutations in LIG4 are associated with disease variants including severe combined immunodeficiency (SCID), highlighting its importance in immune development.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant immunogen spanning amino acids 504-685 of human LIG4, a region within the catalytic core domain. For Western blot, consider reducing conditions and a positive control such as HeLa or Jurkat cell lysates; the expected molecular weight is approximately 104 kDa.
• In ELISA applications, validate antibody specificity using recombinant LIG4 protein or peptide coating, and optimize detection conditions for human samples.
• Given the rabbit origin, use an appropriate anti-rabbit secondary antibody conjugated to HRP or other detection enzymes.
• Cross-reactivity is reported for human; when applying to other species, perform sequence alignment and pilot testing.
• The antibody is packaged and shipped on ice bags; store as recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive portfolio of IVD raw materials, including this DNA Ligase IV antibody for DNA repair and genome stability research. From validated monoclonal antibodies and antibody pairs to bulk ancillary reagents, CamelBio bridges the gap between concept and clinic, supporting every stage of development with reliable sourcing and technical consultation.