Anti-DNA Ligase III/LIG3 Rabbit Polyclonal Antibody for WB, IP, ELISA - P49916

Core Product Specifications and Parameters

Biological Background: DNA Ligase III Function and Localization

• DNA ligase 3 (LIG3), also known as DNA ligase III or polydeoxyribonucleotide synthase [ATP] 3, is an ATP-dependent DNA ligase critical for DNA repair and recombination.
• Isoform 3 functions as a heterodimer with XRCC1 in the nucleus, correcting defective DNA strand-break repair and sister chromatid exchange following ionizing radiation or alkylating agent treatment. Related references: PMID:10207110 PMID:24674627
• Isoform 1 localizes to mitochondria, where it serves as the DNA ligase in mitochondrial base-excision repair. Related references: PMID:10207110 PMID:24674627
• The protein contains an ATP-binding domain, a zinc-finger motif, and a conserved catalytic core, enabling its ligase activity in an ATP- and magnesium-dependent manner.
• LIG3 expression is detected in testis, thymus, prostate, and heart, reflecting tissue-specific roles in DNA maintenance.
• Subcellular localization includes both the nucleus and the mitochondrion, mediated by alternative initiation and a mitochondrial transit peptide in isoform 1.
• Post-translational modifications such as phosphorylation regulate LIG3 activity, and mutations are linked to primary mitochondrial disease.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 113 kDa. Use appropriate protein ladders and reducing conditions; consider validating in human or mouse cell lysates.
• In immunoprecipitation, optimize antibody concentration and washing conditions to capture endogenous LIG3 complexes, noting its interaction with XRCC1 in nuclear extracts.
• ELISA applications may utilize the antibody for direct or indirect detection; ensure coating antigen corresponds to the immunogen region (amino acids 730-1009).
• Since this is a polyclonal antibody, it may recognize multiple isoforms; verify specificity with appropriate controls or knockout cell lines if isoform discrimination is required.

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