Anti-DNA Ligase III/LIG3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49916

Core Product Specifications and Parameters

Biological Background: DNA Ligase III Function and Localization

• DNA ligase 3 (LIG3) exists as multiple isoforms: isoform 3 (nuclear) and isoform 1 (mitochondrial), with alternative names including LIG2, LIG3alpha, and MTDPS20.
• Isoform 3 (nuclear) forms a heterodimer with XRCC1 and participates in DNA strand-break repair and sister chromatid exchange correction following ionizing radiation and alkylating agent damage. Related references: PMID:10207110 | PMID:24674627
• Isoform 1 (mitochondrial) functions as a DNA ligase in mitochondrial base-excision repair (mtBER), critical for maintaining mitochondrial genome integrity. Related references: PMID:10207110 | PMID:24674627
• Subcellular localization is both nuclear and mitochondrial, consistent with its dual roles in genomic and mitochondrial DNA maintenance.
• Predominantly expressed in testis, thymus, prostate, and heart, reflecting tissues with high metabolic or proliferative demands.
• Contains an ATP-binding domain, two zinc-finger motifs, and requires magnesium as a cofactor for its ATP-dependent ligase activity.
• Gene mutations are associated with mitochondrial DNA depletion syndrome 20 (MTDPS20), a primary mitochondrial disease, underscoring its essential mitochondrial function.
• Involved in broader cellular processes including DNA replication, recombination, and cell cycle progression, linking DNA repair to genome stability.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 254–460, a region conserved across both nuclear and mitochondrial isoforms; therefore, the antibody may recognize both forms.
• For Western blotting, anticipate a dominant band near 113 kDa; include appropriate positive controls (e.g., HeLa, HEK293, or testis lysate) and validate in the relevant sample system.
• In IHC-P, consider antigen retrieval methods and tissue-specific expression patterns; testis, thymus, prostate, and heart are suitable positive tissues.
• Cross-reactivity with mouse and rat allows application in common model organisms; however, verify species-specific reactivity in your experimental setup.
• The polyclonal nature may offer robust signal but lot-to-lot consistency should be evaluated for quantitative assays.
• For ELISA and IF/ICC, optimize primary antibody dilution and detection conditions using known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories. This anti-DNA Ligase III antibody supports DNA repair, mitochondrial biology, and disease research; we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, ancillary reagents, and assist in sourcing rare-target raw materials. From concept to clinic, CamelBio is your trusted partner for end-to-end IVD development needs.