Anti-DNA Ligase III/LIG3 Monoclonal Antibody for WB, IHC-P, ELISA - P49916

Core Product Specifications and Parameters

Biological Background: DNA Ligase III/LIG3 Function and Localization

• DNA ligase 3 (LIG3) encodes multiple isoforms: isoform 3 forms a heterodimer with XRCC1 in the nucleus to correct defective DNA strand-break repair and sister chromatid exchange after ionizing radiation and alkylating agents, while isoform 1 is targeted to mitochondria and functions in mitochondrial base-excision DNA repair. Related references: PMID:10207110 PMID:24674627
• The protein contains essential zinc-finger motifs and belongs to the ATP-dependent DNA ligase family, playing key roles in DNA recombination, replication, and repair.
• LIG3 localizes to both the nucleus and mitochondria, supporting its dual function in maintaining genomic and mitochondrial integrity.
• It is a phosphoprotein with regulatory roles during the cell cycle, particularly in cell division and DNA replication.
• High expression is observed in testis, thymus, prostate, and heart, indicating tissue-specific DNA repair demands.
• Mutations in LIG3 are associated with mitochondrial DNA depletion syndrome 20 (MTDPS20), a primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen (aa 400–500) lies within a conserved region; cross-reactivity with mouse and rat is expected based on sequence homology, but validation in the relevant sample system is recommended.
• For Western blotting, expect a band at ~113 kDa; consider using a low-percentage gel and extended transfer times for efficient blotting of high-molecular-weight proteins.
• For IHC-P, optimize antigen retrieval using heat-induced epitope retrieval (e.g., citrate buffer pH 6.0 or EDTA buffer pH 9.0) and validate with appropriate tissue controls.
• ELISA protocols may utilize direct or indirect detection; pairing with a validated detection antibody targeting a different epitope can enhance assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with comprehensive IVD raw material sourcing, from initial concept to clinical application. For DNA repair and mitochondrial biology research, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-LIG3 clone, bulk ancillary reagents, and custom sourcing for rare target raw materials. Contact us to streamline your IVD development pipeline.