Anti-DNA Ligase I Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18858

Core Product Specifications and Parameters

Biological Background: DNA Ligase I Function and Localization

• DNA Ligase I (LIG1) is an ATP-dependent enzyme that seals single-strand breaks (nicks) in double-stranded DNA during DNA repair, replication, and recombination. Related references: PMID:30395541
• The protein is also known as Polydeoxyribonucleotide synthase [ATP] 1 and is encoded by the LIG1 gene located on human chromosome 19.
• Mutations in LIG1 are associated with immunodeficiency disorder IMD96, highlighting its critical role in immune cell development.
• LIG1 localizes to the nucleus, consistent with its function in maintaining genomic integrity.
• The protein contains ATP-binding and magnesium-binding sites, essential for its catalytic activity as a DNA ligase.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity during the cell cycle.
• LIG1 is a reference proteome component, widely studied in DNA damage response mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-91) of human DNA Ligase I; verify epitope accessibility in your assay format.
• For Western blotting, a band around 102 kDa is expected; use appropriate molecular weight markers.
• Due to cross-reactivity with mouse and rat, this antibody may be suitable for comparative studies across species; validate in relevant model systems.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider appropriate fixation and permeabilization protocols to preserve antigenicity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research institutes with validated antibodies like this Anti-DNA Ligase I monoclonal antibody, ideal for DNA repair and cancer research. Beyond single antibodies, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your assay development from concept to clinic.