Anti-DET1 Polyclonal Antibody for WB and ELISA - P48732

Core Product Specifications and Parameters

Biological Background: DET1 Function and Localization

• DET1 (DEETIOLATED 1) is a plant-specific light-mediated development protein that acts as a key repressor of photomorphogenesis in darkness.
• It functions within the CDD complex to regulate ubiquitin conjugating enzymes, promoting ubiquitination and degradation of photomorphogenesis-promoting factors like HY5, HYH, and LAF1, thereby repressing deetiolation. Related references: PMID:9681024, PMID:15342494
• DET1 associates with histone tails and is required for the stability of the CDD complex, indicating a role in chromatin remodeling. Related references: PMID:9681024, PMID:15342494
• It represses blue light-responsive promoters in chloroplasts, linking light signaling to chloroplast gene expression.
• DET1 controls histone H2B monoubiquitination (H2Bub) across most genes by regulating the stability of the deubiquitination module. Related references: PMID:30192741
• Subcellular localization is primarily in the nucleoplasm, consistent with its nuclear functions.
• Involved in the phytochrome signaling pathway and ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• For Western blot detection of DET1 (~62 kDa) in Arabidopsis thaliana lysates, optimize primary antibody dilution (e.g., 1:500–1:2000) and use appropriate loading controls.
• The immunogen (residues 350–450) lies within a conserved region; cross-reactivity with other plant species is not reported—empirical validation is recommended for non-Arabidopsis systems.
• For ELISA, the synthetic peptide immunogen can serve as a coating antigen; include appropriate negative controls to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials tailored for plant biology and developmental signaling research, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This DET1 polyclonal antibody supports investigations into Arabidopsis photomorphogenesis and ubiquitin pathways. Our integrated supply chain and technical support accelerate your assay development from concept to clinic.