Anti-CYP7B1 Polyclonal Antibody for WB, IF/ICC, ELISA - O75881

Core Product Specifications and Parameters

Biological Background: CYP7B1 Function and Localization

• CYP7B1 is a cytochrome P450 monooxygenase that catalyzes the 7-alpha hydroxylation of oxysterols and steroids, utilizing molecular oxygen and electrons from NADPH via cytochrome P450 reductase (PubMed:10588945, PubMed:24491228) Related references: PMID:10588945, PMID:24491228
• It hydroxylates 25-hydroxycholesterol and 26-hydroxycholesterol to produce 7-alpha,25-dihydroxycholesterol, a ligand for the G protein-coupled receptor GPR183/EBI2, thereby regulating B cell migration in germinal centers and humoral immunity (PubMed:10588945, PubMed:9802883) Related references: PMID:10588945, PMID:9802883
• CYP7B1 also metabolizes neurosteroids like dehydroepiandrosterone and pregnenolone, which are implicated in hippocampus-associated memory and learning (PubMed:24491228) Related references: PMID:24491228
• The enzyme is predominantly localized to the endoplasmic reticulum membrane and microsomal membrane.
• It is widely expressed with highest levels in brain, testis, ovary, liver, kidney, small intestine, thymus, and spleen.
• Mutations in CYP7B1 are linked to hereditary spastic paraplegia type 5A (SPG5A) and congenital bile acid synthesis defect 3 (CBAS3), highlighting its critical role in cholesterol catabolism and neuronal maintenance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human CYP7B1, which is conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blotting, the predicted molecular weight is 58 kDa. Validate with positive control lysates from liver or brain tissue that endogenously express CYP7B1.
• For immunocytochemistry/immunofluorescence, ensure adequate permeabilization to detect the ER membrane-localized protein. Include organelle markers for co-localization studies.
• In ELISA applications, consider testing the antibody as a capture or detection reagent in a sandwich pair after in-house optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies for metabolic and neurological targets. For CYP7B1-related projects — such as oxysterol profiling, neurosteroid biosynthesis, or cholestasis research — we offer polyclonal antibodies, antibody pairs, and ancillary reagents. Our sourcing capabilities extend to rare targets and bulk quantities, facilitating seamless development from bench to clinic.