Anti-CYP1A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P04798

Core Product Specifications and Parameters

Biological Background: CYP1A1 Function and Localization

• CYP1A1 encodes a cytochrome P450 monooxygenase that metabolizes endogenous substrates such as fatty acids, steroid hormones, and vitamins via NADPH-dependent oxidation. Related references:
• PMID:10681376
• PMID:11555828
• PMID:12865317
• Exhibits high catalytic activity for estrogen hydroxylation (E1 and E2) at C-2, C-15α, and C-16α positions. Related references:
• PMID:11555828
• PMID:12865317
• PMID:14559847
• Displays regioselective and stereoselective epoxidation of polyunsaturated fatty acids like arachidonic acid and eicosapentaenoic acid. Related references:
• PMID:15041462
• PMID:19965576
• PMID:20972997
• Involved in all-trans retinoic acid biosynthesis via two-step oxidation of retinol. Related references:
• PMID:10681376
• May convert hydroperoxide species into oxo metabolites in a lipoxygenase-like, NADPH-independent reaction. Related references:
• PMID:21068195
• Localizes to the endoplasmic reticulum membrane, mitochondrial inner membrane, microsome membrane, and cytoplasm.
• Tissue expression is reported in lung, lymphocytes, and placenta.
• Post-translational modifications include glycosylation; the enzyme contains heme and iron cofactors and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–460 of human CYP1A1, a region conserved in mouse and rat; cross‑reactivity with these species is expected but should be verified experimentally.
• For Western blot, an endogenous band near 58 kDa is anticipated; validate sensitivity in cell lines or tissues with known CYP1A1 expression (e.g., lung, placenta).
• For immunofluorescence/ICC, appropriate fixation and permeabilization conditions must be optimized for the chosen sample type; consider organelle‑specific markers to confirm subcellular localization.
• ELISA applications may require titration of both capture and detection antibodies; use a standard curve with recombinant CYP1A1 protein for quantitation.
• As a polyclonal reagent, lot‑to‑lot consistency should be monitored; include positive and negative controls in every assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop supply partner for IVD raw materials, supporting diagnostic developers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CYP1A1‑related metabolism and toxicology research, we deliver recombinant‑immunogen‑based polyclonal antibodies and can assist with sourcing rare‑target raw materials, ensuring a streamlined path from concept to clinic.