Anti-Cyclin E2 Monoclonal Antibody for WB, ELISA - O96020

Core Product Specifications and Parameters

Biological Background: Cyclin E2 Function and Localization

• Cyclin E2 is a G1/S-specific cyclin that plays a critical role in cell cycle progression.
• It is encoded by the CCNE2 gene (Gene ID: 9134) and is essential for the transition from G1 to S phase.
• The protein localizes to the nucleus, consistent with its role in cell cycle regulation.
• Tissue expression is highest in testis, thymus, and brain, with lower levels in placenta, spleen, and colon; elevated levels are observed in tumor-derived cells.
• Cyclin E2 is subject to phosphorylation, as indicated by its inclusion in the phosphoprotein keyword, suggesting regulatory mechanisms.
• The molecular weight is approximately 47 kDa (UniProt: 46757 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-150 of human Cyclin E2, which may confer specificity for detecting the native protein in applications like WB.
• For Western blot, consider using human cell lines known to express Cyclin E2, such as HeLa or MCF-7, as positive controls; the predicted molecular weight is ~47 kDa.
• In ELISA, this monoclonal antibody can be explored as a capture or detection reagent; optimization of coating buffer and antibody concentration is recommended.
• Store at -20°C or as recommended upon arrival; avoid repeated freeze-thaw cycles to maintain stability.
• Cross-reactivity is reported for human Cyclin E2; for other species, additional validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For researchers studying cell cycle regulation, CamelBio provides reliable access to anti-Cyclin E2 antibodies and related reagents, streamlining your path from concept to clinic. Our sourcing expertise extends to rare targets, ensuring you have the materials needed for diagnostic development.