Anti-CLTB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P09497

Core Product Specifications and Parameters

Biological Background: CLTB Function and Localization

• Clathrin light chain B (CLTB) is a subunit of clathrin, the major protein component of the polyhedral coat surrounding coated pits and vesicles involved in intracellular trafficking.
• As a core structural component, clathrin forms a triskelion shape that assembles into a lattice-like cage, driving vesicle formation at the plasma membrane and trans-Golgi network.
• CLTB is localized to cytoplasmic vesicle membranes and the membrane of coated pits, consistent with its role in endocytosis and vesicle transport.
• The protein undergoes several post-translational modifications, including acetylation and phosphorylation, and contains calcium-binding sites and disulfide bonds that may regulate clathrin assembly.
• Alternative splicing of CLTB generates multiple isoforms, potentially contributing to functional diversity in different tissues or cellular contexts.
• CLTB has been identified in large-scale proteomics studies and is part of the UniProt reference proteome, underscoring its fundamental cellular role.
• As a clathrin light chain, CLTB is reported to modulate clathrin assembly and disassembly, influencing the dynamics of coated vesicle formation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-211 of human CLTB. Users may consider epitope mapping if precise binding regions are critical for their application.
• For Western blot (WB), a band at approximately 25 kDa is expected. Validate the antibody in the relevant species and sample type, and consider using appropriate positive controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols, as epitope accessibility may be affected. Co-staining with clathrin heavy chain or organelle markers can help confirm localization.
• For ELISA, the antibody may be used as a capture or detection reagent. Titration experiments are recommended to determine optimal working concentrations.
• Cross-reactivity is reported for human, mouse, and rat. If using other species, sequence homology analysis and preliminary testing are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on clathrin-mediated endocytosis and vesicle trafficking, this anti-CLTB rabbit polyclonal antibody serves as a reliable tool. Beyond this product, CamelBio supports rare target raw-material sourcing and technical consultation, facilitating development from concept to clinic.