Anti-Cleaved Caspase-1 p20 Rabbit Monoclonal Antibody for WB, ELISA - P29466

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1 is a thiol protease belonging to the caspase family, encoded by the CASP1 gene (also known as IL1BC, ICE). It is synthesized as an inactive zymogen (p45) that is proteolytically processed into active p20 and p10 subunits.
• Plays a central role in inflammatory responses by cleaving the precursor forms of interleukin-1 beta (pro-IL1B) and interleukin-18 (pro-IL18) into their biologically active mature cytokines, following assembly of inflammasome complexes. Related references: PMID:15326478, PMID:15498465, PMID:1574116
• Additionally, caspase-1 initiates pyroptosis, a lytic programmed cell death, by cleaving Gasdermin-D (GSDMD), generating an N-terminal fragment that forms membrane pores. Related references: PMID:26375003, PMID:32051255, PMID:32109412, PMID:32553275
• During bacterial infection, caspase-1 cleaves and activates caspase-7 (CASP7), contributing to plasma membrane repair. Related references: PMID:22464733
• In antiviral immunity, caspase-1 controls DNA virus-induced responses by cleaving and inactivating cyclic GMP-AMP synthase (CGAS). Related references: PMID:28314590
• Subcellular localization includes the cytoplasm and cell membrane, consistent with its role in processing cytokines and executing pyroptosis.
• Tissue expression is broad, with higher levels in spleen and lung, and detectable amounts in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney, and testis; expression is absent in the brain.
• The protein is subject to alternative splicing and isopeptide bond formation, and its activation involves intermolecular autoproteolytic processing; it belongs to the peptidase C14A family.

Experimental Guidance and Technical Tips

• The immunogen for this monoclonal antibody is a synthetic peptide corresponding to amino acids 200–300 within the p20 subunit of human caspase-1. This region is predicted to be accessible in the cleaved, active form of the enzyme.
• For Western blot (WB) analysis, prepare cell or tissue lysates under reducing conditions. Consider using known inducers of caspase-1 activation (e.g., NLRP3 inflammasome activators such as nigericin or ATP) to observe the cleaved p20 fragment. A band of approximately 20 kDa is expected alongside potential full-length or intermediate pro-forms.
• For ELISA applications, the antibody may be used for capture or detection of cleaved caspase-1 p20 in human serum, plasma, or cell culture supernatants; optimal dilution and pairing should be determined empirically.
• Cross-reactivity with other species has not been reported; validate the antibody in the relevant sample systems if species other than human are targeted.
• Given the multiple protein weights reported (10/29/35/42/45 kDa), these likely represent different cleavage intermediates and isoforms; confirm the identity of detected bands using appropriate controls (e.g., untreated vs. activated samples).

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CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this anti-cleaved caspase-1 p20 antibody to advance research in inflammation, immunology, and programmed cell death. Beyond antibodies, we supply a full spectrum of ancillary reagents, antigen proteins, and customized bulk production to accelerate your diagnostic development from concept to commercialization. Our expertise in rare target sourcing ensures you get consistent quality materials for all your assay needs.