Anti-CHAT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P28329

Core Product Specifications and Parameters

Biological Background: CHAT Function and Localization

• CHAT, or Choline O-acetyltransferase, catalyzes the reversible synthesis of acetylcholine from acetyl-CoA and choline at cholinergic synapses, playing a pivotal role in neurotransmitter biosynthesis.
• The protein is known by several disease-associated aliases, including CMS6, CMS1A, and CMS1A2, linking it to congenital myasthenic syndromes, particularly those with episodic apnea.
• Keywords from the UniProt record classify CHAT as a phosphoprotein with alternative splicing, suggesting regulatory post-translational modifications and isoform diversity.
• It is annotated with acyltransferase and transferase activities, placing it within a family of enzymes critical for metabolic and signaling pathways.
• Structural investigations (3D-structure keyword) and direct protein sequencing have provided insights into its molecular architecture and functional domains.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200-300 of human CHAT; this region may be conserved across species (human, mouse, rat), so cross-reactivity is expected as indicated.
• For Western blotting (WB), expect a band around 83 kDa; due to alternative splicing, multiple isoforms may be detected. Validate with relevant positive controls (e.g., brain lysate).
• For immunohistochemistry (IHC-P), consider standard antigen retrieval methods; staining is anticipated in cholinergic neurons but should be optimized in your tissue of interest.
• In ELISA, the polyclonal nature may increase sensitivity; however, optimize antibody dilution and blocking conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive one-stop platform for sourcing IVD raw materials, including validated antibodies and ancillary reagents. This CHAT antibody supports neuroscience and cholinergic signaling research, and we can assist with bulk purchases, customized panels, or rare target reagent procurement to streamline your development pipeline. Contact us to discuss your unique requirements.