Anti-CARS Polyclonal Antibody (Rabbit) for WB, IF/ICC, ELISA - P49589

Core Product Specifications and Parameters

Biological Background: CARS Function and Localization

• CARS (cysteine--tRNA ligase, cytoplasmic) is a class I aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of cysteine to its cognate tRNA(Cys), an essential step in protein biosynthesis.
• Also known as cysteinyl-tRNA synthetase (CysRS), it plays a critical role in decoding the genetic code by charging tRNA(Cys) with cysteine.
• The enzyme localizes predominantly to the cytoplasm, consistent with its function in cytosolic protein synthesis.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity.
• CARS has been associated with disease variants linked to dwarfism and chromosomal rearrangements, and is annotated as a proto-oncogene.
• The protein contains conserved motifs for ATP-binding, metal-binding (zinc), and nucleotide-binding, characteristic of its enzymatic function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 469–748) of human CARS. Antibody specificity should be validated using appropriate positive and negative controls.
• For Western blotting (WB), the predicted molecular weight is 85kDa. A specific band at ~85kDa is expected in human and mouse samples; consider using reducing conditions.
• In immunofluorescence/ICC, a cytoplasmic staining pattern is anticipated. Fixation and permeabilization conditions may require optimization.
• For ELISA applications, titer and working concentration should be determined empirically using the immunogen or native protein.
• Cross-reactivity with human and mouse has been reported; reactivity with other species should be individually verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research labs, offering a comprehensive portfolio including polyclonal antibodies like this CARS antibody for protein translation and tRNA synthetase studies. Our supply capabilities cover validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents, ensuring you have the right tools from concept to clinic. Whether you need routine targets or rare raw materials, our sourcing solutions support your workflow with reliability and expertise.