Anti-BRD9 Rabbit Monoclonal Antibody for WB, ELISA - Q9H8M2

Core Product Specifications and Parameters

Biological Background: BRD9 Function and Localization

• BRD9 (Bromodomain-containing protein 9), also known as Rhabdomyosarcoma antigen MU-RMS-40.8, plays a role in chromatin remodeling and regulation of transcription. Related references: PMID:22464331, PMID:26365797
• It acts as a chromatin reader that specifically recognizes and binds acylated histones, including those that are acetylated and butyrylated. Related references: PMID:26365797
• BRD9 is a core component of the GBAF (GLTSCR1/1L-containing BAF) subcomplex of the SWI/SNF chromatin remodeling complex, which uses ATP hydrolysis to modulate DNA-histone contacts within nucleosomes. Related references: PMID:29374058
• It contributes to DNA repair by facilitating the formation of the RAD51-RAD54 complex, thereby promoting homologous recombination. Related references: PMID:32457312
• The protein is predominantly localized in the nucleus, consistent with its role in chromatin-associated processes.
• Post-translational modifications of BRD9 include acetylation, phosphorylation, and ubiquitination, which may regulate its activity and interactions.
• Multiple isoforms are generated by alternative splicing, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning the C-terminal region (aa 274–597) of human BRD9, which encompasses the bromodomain. Given the high conservation of this domain, cross-reactivity with mouse and rat is observed.
• For Western blotting, a band near 67 kDa is expected. Using nuclear extracts from human, mouse, or rat cell lines may enhance detection due to the nuclear localization of BRD9.
• In ELISA applications, the antibody can be considered as a capture or detection reagent; optimization with recombinant BRD9 protein or cell lysates is advised.
• Always validate the antibody in the relevant sample system and perform appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-BRD9 monoclonal antibody supports research in chromatin remodeling, transcription regulation, and DNA repair. From rare targets to custom needs, CamelBio streamlines procurement to accelerate your diagnostic development from concept to clinic.