Anti-Alpha-Fetoprotein (AFP) Monoclonal Antibody for WB, IF/ICC, ELISA - P02771

Core Product Specifications and Parameters

Biological Background: Alpha-Fetoprotein (AFP) Function and Localization

• Alpha-fetoprotein (AFP), also known as alpha-1-fetoprotein or alpha-fetoglobulin, is a fetal glycoprotein belonging to the albumin family, characterized by internal repeat structures.
• AFP serves as a transport protein for copper, nickel, fatty acids, and bilirubin, with lower bilirubin affinity compared to serum albumin.
• A minor fraction (less than 2%) of human AFP displays estrogen-binding properties.
• The protein is secreted and found in plasma, primarily synthesized by the fetal liver and yolk sac during development.
• AFP undergoes post-translational modifications including phosphorylation, sulfation, and N-linked glycosylation, and contains disulfide bonds.
• Its molecular weight is approximately 69 kDa (observed on SDS-PAGE) with a calculated mass of 68.7 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against recombinant full-length human AFP; thus, it may recognize native and denatured conformations depending on the application.
• For Western blot, consider using a reduced/denatured sample preparation, as the predicted molecular weight is 69 kDa.
• In IF/ICC, validate fixation and permeabilization conditions to ensure antibody access, as AFP is a secreted protein.
• Cross-reactivity with mouse and rat AFP is reported; however, verify performance in the relevant species’ sample system.
• For ELISA, use appropriate coating and detection conditions, and titrate the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive supplier of IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-AFP antibody supports liver cancer biomarker research and diagnostic development. With an end-to-end supply and technical service model, CamelBio accelerates your path from concept to clinic.