Anti-ABI3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9P2A4

Core Product Specifications and Parameters

Biological Background: ABI3 Function and Localization

• ABI3 (ABI gene family member 3), also known as NESH (New molecule including SH3) and SSH3BP3, belongs to the ABI protein family characterized by the presence of an SH3 domain and a coiled-coil region.
• It is involved in the regulation of cell motility and may function as a tumor metastasis suppressor, as indicated by in vitro studies showing reduced cell migration.
• ABI3 localizes primarily to the cytoplasm, consistent with a role in modulating cytoskeletal dynamics and intracellular signaling pathways.
• The protein contains an SH3 domain (Src homology 3) which typically mediates protein-protein interactions, and a coiled-coil domain, suggesting involvement in oligomerization or complex formation.
• According to UniProt, ABI3 is a phosphoprotein and has been identified in proteomics analyses, indicating it may be regulated by phosphorylation and is expressed under physiological conditions.
• ABI3 undergoes alternative splicing, which could generate multiple isoforms with potentially distinct functions or tissue distributions.
• Tissue expression profiling shows ABI3 is broadly expressed, with detectable levels in heart, lung, liver, pancreas, kidney, and placenta, and lower levels in brain and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region of ABI3 (a.a. 197-366); this design may confer specificity for full-length ABI3 and potentially detect splice variants containing this segment.
• For Western blotting (WB), use appropriate lysis buffer with protease and phosphatase inhibitors, and consider the predicted molecular weight of ~39 kDa when assessing band size.
• In immunohistochemistry on paraffin-embedded tissues (IHC-P), optimize antigen retrieval conditions; tissues with known ABI3 expression such as pancreas or placenta can serve as positive controls.
• When performing ELISA, match the coating antigen to the immunogen region or validate detection of native protein in the relevant sample matrix.
• Cross-reactivity with mouse and rat ABI3 has been confirmed; however, for quantitative applications, validation in the target species and sample type is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement gateway to high-quality IVD raw materials like this anti-ABI3 rabbit monoclonal antibody, accelerating your oncology and cell motility research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring you have all components for robust diagnostic assays. Partner with us to efficiently bridge discovery and clinical application with end-to-end supply chain and technical consultation.