Anti-ABCE1 Rabbit Polyclonal Antibody for WB and ELISA - P61221

Core Product Specifications and Parameters

Biological Background: ABCE1 Function and Localization

• ABCE1 is a nucleoside-triphosphatase that hydrolyzes ATP, GTP, UTP, and CTP, driving ribosome disassembly into free 60S and 40S subunits after translation termination or ribosome stalling.
  Related references:
  PMID:20122402, PMID:21448132

• It plays a critical role in the No-Go Decay (NGD) pathway by disassembling stalled ribosomes in cooperation with the Pelota-HBS1L complex.
  Related references:
  PMID:21448132

• ABCE1 participates in mitochondrial quality control; upon mitochondrial damage, it is ubiquitinated by CNOT4, generating polyubiquitin signals that trigger mitophagy.
  Related references:
  PMID:29861391

• As an RNase L inhibitor, it antagonizes the binding of 2-5A to RNASEL, thereby down-regulating the interferon-regulated antiviral 2-5A/RNASEL pathway.
  Related references:
  PMID:9660177, PMID:11585831, PMID:9847332

• During viral infection, ABCE1 may act as a chaperone for HIV-1 capsid assembly and facilitates down-regulation of the 2-5A/RNASEL pathway in EMCV and HIV-1 infections.

• The protein is primarily localized in the cytoplasm but is also found in mitochondria, consistent with its dual roles in translation and mitochondrial homeostasis.

• ABCE1 is a 67 kDa member of the ATP-binding cassette (ABC) transporter family, containing conserved nucleotide-binding domains and a 4Fe-4S cluster.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 320–599 of human ABCE1, a region likely to generate antibodies that recognize the native folded protein with high specificity.
• For Western blot, a band of approximately 67 kDa is expected in human and mouse cell lysates. Optimize loading amounts and antibody dilution empirically.
• ELISA setups may benefit from coating with recombinant ABCE1 protein; consider both direct and capture formats.
• The antibody cross-reacts with mouse, making it suitable for translational studies in rodent models; validate in the relevant sample system.
• Given ABCE1’s role in ribosome recycling, consider using polysome profiling or puromycin incorporation assays to confirm functional impact if using the antibody for co-immunoprecipitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with one-stop access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For projects involving ABCE1—a key player in translation, mRNA surveillance, and antiviral pathways—our portfolio includes reagents like this rabbit pAb, complemented by technical consulting and custom sourcing services for rare targets. Whatever your development stage, CamelBio streamlines the path from concept to clinic with reliable supply and expert support.