ZSCAN4C Rabbit pAb - 245109

Core Product Specifications and Parameters

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from mouse tissues or cell lines known to express ZSCAN4. A band around 57 kDa is expected. Optimize primary antibody dilution and validate results with appropriate positive and negative controls, such as ZSCAN4 knockout samples.
• For ELISA, the antibody can be used as a capture or detection reagent. Pair with a suitable matched antibody for sandwich ELISA and titrate against recombinant ZSCAN4 or cell lysates to determine optimal concentrations.
• The immunogen is a C-terminal peptide (aa 400-506); therefore, the antibody may detect both full-length and C-terminal fragments. Cross-reactivity is confirmed for mouse; testing with other species requires empirical validation.

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