ZSCAN4 Rabbit Polyclonal Antibody for WB and ELISA - Q8NAM6

Core Product Specifications and Parameters

Biological Background: ZSCAN4 Function and Localization

• ZSCAN4, also known as zinc finger protein 494 (ZNF494), is a member of the zinc finger and SCAN domain-containing protein family.
• It functions as an embryonic stem (ES) cell-specific transcription factor essential for the maintenance of pluripotency.
• ZSCAN4 binds directly to telomeres and plays a critical role in preserving genomic stability in ES cells by regulating telomere elongation.
• It acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE), a mechanism that contributes to telomere lengthening in undifferentiated ES cells.
• The protein localizes to the nucleus, specifically associating with chromosome telomeres.
• ZSCAN4 contains multiple zinc finger motifs, indicating its capacity for DNA binding and metal ion coordination.
• Key biological keywords include: DNA-binding, transcription regulation, telomere maintenance, and metal-binding.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1-227 of human ZSCAN4, covering the N-terminal region. For ELISA, consider coating with the immunogen or a similar recombinant protein to ensure specificity.
• For Western blot analysis, the predicted molecular weight is approximately 49 kDa. Use lysates from human embryonic stem cells or ZSCAN4-overexpressing cell lines as positive controls; validate in your relevant experimental system.
• As a rabbit polyclonal antibody, it may exhibit lower lot-to-lot consistency compared to monoclonals; we recommend titrating the antibody for each new lot and application.
• Optimize primary antibody dilutions for WB and ELISA; typical starting dilutions for polyclonals in WB range from 1:500 to 1:2000, but empirical determination is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined channel to source validated IVD raw materials such as this ZSCAN4 antibody, which supports investigations into stem cell pluripotency and telomere biology. Beyond individual antibodies, we offer integrated access to optimized monoclonal and polyclonal antibody pairs, bulk ancillary reagents, and rare target sourcing—empowering your assay development from concept to clinic.