ZPR1 Rabbit Polyclonal Antibody for WB and ELISA - O75312

Core Product Specifications and Parameters

Biological Background: ZPR1 Function and Localization

• ZPR1 (Zinc finger protein ZPR1, also designated ZNF259) is a zinc finger protein that functions as a signaling molecule communicating proliferative growth signals from the cytoplasm to the nucleus, thereby positively regulating cell cycle progression.
  Related references:
  PMID:29851065
• It plays a key role in the localization and accumulation of the survival motor neuron protein SMN1 in sub-nuclear bodies, including gems and Cajal bodies, and is essential for SMN1-mediated functions.
• ZPR1 induces neuron differentiation, stimulates axonal growth, and promotes growth cone formation in spinal cord motor neurons, highlighting its importance in neuronal development.
• The protein is involved in the splicing of cellular pre-mRNAs, contributing to mRNA processing and maturation.
• ZPR1 may be implicated in H2O2-induced neuronal cell death, suggesting a role in oxidative stress responses.
• Subcellularly, ZPR1 localizes to the nucleus, nucleolus, gems, and Cajal bodies, as well as the cytoplasm, perinuclear region, and neuronal projections including axons and growth cones.
• Expression of ZPR1 was detected in fibroblasts, with notably weaker expression in fibroblasts derived from spinal muscular atrophy (SMA) patients.
• The protein contains zinc finger domains, classifying it as a metal-binding, repeat-containing protein, and is associated with diseases such as deafness, dwarfism, and hypotrichosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110–459 of human ZPR1 (central region), likely generating antibodies recognizing multiple epitopes; consider verifying antibody specificity using full-length recombinant protein or cell lysates with knockdown validation.
• For Western blot (WB) applications, the predicted molecular weight of ZPR1 is approximately 51 kDa; note that actual migration may differ due to post-translational modifications or isoform variations—optimize gel percentage and transfer conditions accordingly.
• For ELISA, the recombinant protein immunogen can serve as a coating antigen; however, confirm that the antibody recognizes the native folded protein if targeting endogenous ZPR1 in complex biological matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For ZPR1-related research, particularly in cell signaling, neurodegenerative diseases like SMA, and neuronal differentiation, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert assistance in sourcing rare targets. Let us streamline your raw-material procurement so you can focus on accelerating your IVD development.